By Donald J. Hanahan

ISBN-10: 0195079809

ISBN-13: 9780195079807

ISBN-10: 0195079817

ISBN-13: 9780195079814

This publication offers a concise creation to phospholipid chemistry and is meant for a wide viewers of biologists, biochemists, and graduate scholars. constructed as a part of a graduate path on lipids, this publication additionally serves as a reference for laboratory investigators on sign transduction and organic membranes. the 1st a part of the textual content is dedicated to an orientation to the chemical nature of lipids commonly, how they're considered linked within the telephone, and the technique in which the mobile lipids (including the phospholipids) could be recovered from cells and subjected to an preliminary id. next chapters signify the choline-containing phospholipids, together with the sphingolipids, the non-choline containing phospholipids, and at last, the so-called minor phospholipids. The latter compounds, which act as agonists or lipid chemical mediators on cells, shape a leading edge of a brand new type of biologically energetic ingredients and feature set the research of mobile phospholipids on a brand new and exiting path. most significantly, this publication presents a foundation for extra inquiry on those complex molecules, displaying that even supposing the compounds are designated, with care and realizing, they are often studied conveniently

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Extra info for A Guide to Phospholipid Chemistry

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While there is no doubt that one must remove these nonlipid substances so that highly purified lipid may be obtained for particular study, it is interesting to reflect on another aspect of the problem. On the one hand, it is entirely conceivable that the nonlipid components are simply adventitious contaminants and bear no relation to the lipid behavior as such. On the other hand, there is developing evidence that substances, such as divalent cations as well as carbohdyrates, may have close associations with lipids of a tissue, and these interactions might have an important metabolic consequences.

Additional associations can derive from polar and charged groups, such as the carboxyl or phosphate residues. Also, steric fit must play a role and water must be considered as an important participant. The ability of lipids (such as phospholipids) to swell in water and for proteins to bind definitive quantities of water has been well established for many years. Thus, water must be considered as an important constituent of any lipid-protein complex, whether it is a soluble plasma constituent or an insoluble component found in membranes.

5 cm above the bottom edge. The (top) lid is placed securely on the chromatography tank, and the solvent is allowed to ascend up the plate to within 1-2 cm from the top. In this solvent system and with these silica gel plates, the time for this operation is close to 50 min. The plates are then removed from the tank, and the solvent front is quickly marked on each lengthwise edge and placed in a fume hood to dry. These are the rudimentary steps to take for a single-dimensional thin-layer chromatography.

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A Guide to Phospholipid Chemistry by Donald J. Hanahan

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